Prerequisite(s): BIOL 230 with a grade of C- or higher or departmental approval. An introduction to biological applications of microscopy. Topics include history of microscopy, preparation of samples for light and electron microscopy, phase, polarization, interference, confocal, and cryogenic microscopy, as well as transmission and scanning electron microscopy. Special attention will be paid to microscopic localization and dynamics of biological molecules plus ions and intracellular messengers.