Advanced Molecular Microbiology course will address concepts that are essential for understanding of the molecular biology of microorganisms: environmental sensing and signal transduction pathways; regulation of gene expression: transcription, bacterial operons, and post-transcriptional regulatory mechanisms; bacterial cell division and its regulation, bacterial cell wall biosynthesis, mechanisms of adherence and invasion, molecular mechanisms of bacterial persistence and antibiotic resistance. This course provides students with opportunities to study advanced methods of genetic engineering: DNA cloning, the nature, selection and design of DNA cloning vectors, restriction enzymes, modifying enzymes, polymerases, bacterial transformation, Western blot, and other tools and techniques used in molecular biology. Some aspects of bioinformatics and genomics, as well as other advanced molecular technologies will be discussed. Overall this course will aid understanding of fundamental aspects of the molecular biology of microorganisms as they relate to biomedical sciences.